Creative Bioarray is committed to building a 3D tumor model that accurately reproduces the tumor phenotype in vivo through cell patterning technology, so as to simulate the tumor behavior and drug resistance encountered in vivo, provide a new and effective tool for the study of tumor tissue engineering, and allow you to conduct better preclinical evaluation of anticancer drug candidates.
Tumor microenvironment is a local microenvironment composed of tumor cells, stromal cells, extracellular matrix and a variety of factors. It plays an important role in the process of tumor survival, growth, invasion and metastasis. At present, it has become a new target of tumor therapy. In the tumor microenvironment, hypertonic tumor blood vessels increase interstitial fluid pressure and change the flow pattern. The abnormal fluid pressure can promote tumor angiogenesis and promote tumor growth by stimulating tumor cells and endothelial cells.
Through microfluidic cell patterning, we can co-culture tumor cells and endothelial cells in vitro, so as to explore the effects of different flow shear stress on tumor angiogenesis, and easily solve the problem of accurate control of fluid pressure in the culture environment. In addition, cell patterning has the advantages of miniaturization, flexible design, easy integration, real-time observation and high throughput. Therefore it is easier to establish in vitro models close to the real tumor microenvironment in vivo, including simulating tumor angiogenesis, tumor invasion and metastasis. We can provide an effective way to further study the molecular mechanism of tumor microenvironment and develop new drugs.
The characteristics of tumor blood vessels are often not considered in the traditional in vitro angiogenesis model, but our microfluidic cell patterning technology can simulate and restore the characteristics of tumor blood vessels by virtue of its own advantages. Compared with other models, the advantage of the in vitro tumor model established by this technology is that the interface between cancer tissue and blood vessels can be accurately imaged during tumor angiogenesis, and the angiogenesis response and cross endothelial migration of tumor cells can be quantitatively analyzed.
We can construct tumor vascular microfluidic chip by cell patterning technology. The chip can not only control the flow direction and velocity of the liquid in the microchannel and realize the simulation of the changeable characteristics of the blood flow direction and velocity in the tumor blood vessel, but also adjust the flow direction and velocity of the microchannel pipe in segments, simulate the distorted and uneven thickness of the tumor blood vessel structure, and even make the blood vessel appear stable leakage points by promoting the migration of endothelial cells, so as to reproduce the characteristics of high permeability of tumor blood vessels.
At present, traditional cell co culture, such as 2D co-culture on the surface of pore plate, can't represent the tissue environment including cell-cell and cell-matrix interaction, and can't reproduce the structural, tissue or pathophysiological characteristics of cancer. 3D co-culture allows single cell to maintain normal morphology, structure and energy supply, controls the interaction between cells, simulates a more natural microenvironment, and avoids many defects of 2D co culture.
We can realize the 3D co-cultures of tumor cells and a variety of cells by cell patterning, and make contributions to the study of tumor matrix interaction, intravascular infiltration and angiogenesis. Our microfluidic cell patterning can realize the adjacent culture of cancer cells and stromal cells, and is also suitable for the encapsulation of tumor aggregates in the extracellular matrix. It can fill the cells with matrix glue, so as to simulate the microenvironment of cells in vivo, and provide sufficient areas for the interaction between cells. Therefore, the 3D tumor cell co-culture models established by our microfluidic cell patterning can allow the interaction between components in the tumor microenvironment to study physiological related conditions in vitro.
Creative Bioarray provides customers with cell patterning customization and related detection services based on Cell Patterning, you can contact our employees directly to ask questions if you are interested in our services, please contact us for more details.