In vivo, hepatocytes are organized into highly complex structures. There is a good interaction between non parenchymal cells and extracellular matrix, so that hepatocytes can maintain their physiological responsibilities. Using standard cell culture methods, hepatocytes were cultured in flat dishes. This static 2D cell culture system showed poor cell viability, and the cells lost their phenotypic function.
Our cell patterning technology is one of the best technologies to accurately pattern heterogeneous hepatocytes to simulate biological liver morphology and maintain hepatocyte function. It can simulate the in vivo microenvironment of cells, maintain good cell-cell and cell ECM interaction, optimal oxygen and nutrient supply, accurately control temperature and pH value, biochemical and mechanical stress and many other factors, so as to achieve a functional in vitro liver model with high cell viability. This technology establishes a culture model for drug metabolism / toxicity screening for basic researchers in drug discovery and liver regeneration, disease, pathophysiology and pharmacology.
Liver tissue with complete function includes many types of cells. The function of hepatocytes is related to promoting the interaction of hepatocyte-hepatocyte, hepatocyte-ECM and hepatocyte-NPC. The traditional hepatocyte expansion culture model can't simulate the microstructure of normal liver tissue, and the lack of interaction leads to the rapid loss of hepatocyte specific biological activity.
The culture system established by our cell patterning technology can bring a variety of cell types, including hepatic parenchymal cells and hepatic non parenchymal cells, into hepatocyte culture, and show higher hepatocyte like function by initiating the interaction between different hepatocytes. Compared with traditional 2D culture, all these cells can maintain their morphology by proliferating in 3D system.
As a very attractive source of hepatocytes, stem cells have appropriate compatibility with the host immune system, and have great prospects in liver tissue engineering and regenerative medicine.
Our cell patterning technology can accurately control the cell microenvironment to enhance the function of primary human hepatocytes and human hepatocyte like cells derived from induced pluripotent stem cells, realize the co culture of liver derived or non-liver derived non parenchymal cell types, promote the differentiation of stem cells into liver phenotypes, induce a significant increase in liver expression, and maintain the long-term function of hepatocytes (more than 4 weeks) stable.
Vascularization is crucial in tissue engineering because blood vessels are needed to provide nutrition and oxygen to tissues and transport waste. Through cell patterning technology, a three-dimensional vascular functional system suitable for hepatocyte growth in vitro can be constructed to fully supply nutrients and oxygen, so as to realize the high activity culture of hepatocyte aggregates in vitro and the coordinated growth of multiple cells. At the same time, many parameters can be controlled by using this technology, including maintaining high cell density to facilitate cell-cell and cell ECM interaction, appropriate cell inoculation to reduce cell damage, good perfusion rate to provide sufficient nutrition and sufficient oxygen supply, so as to promote angiogenesis.
The vascularized liver organoids constructed by our cell patterning technology can closely imitate the natural mode of the liver and show improved liver function, metabolic activity, biosynthetic activity and drug response.
Creative Bioarray provides customers with cell patterning customization and related detection services based on Cell Patterning, you can contact our employees directly to ask questions if you are interested in our services, please contact us for more details.