Creative Bioarray provides you with the construction service of in vitro vascularized tissue based on the Cell Patterning. The models can be used for in vitro research, drug screening and high-throughput detection in clinical trials of diseases such as cancer or ischemia (insufficient blood supply to organs or tissues), so as to reduce the cost of drug development and provide important preclinical information.
Vascularization is one of the most important factors to determine whether the engineering structure can successfully maintain its vitality, because it ensures the nutrition and oxygen supply of metabolic cells. However, the construction of vascular network has always been a challenge in the field of tissue engineering. Cell patterning technology has become a promising tool, which can replicate the structure of vascularized tissues in vitro, including a series of branching vessels, from millimeter sized small diameter vessels to micron sized capillary networks supporting tissue-specific functions. This technology can provide a cell-based long-term and stable solution for supplying oxygen and nutrition to tissues, which is essential for the sustainable growth and survival of tissues.
Fig1. Schematic diagram of diffusion and transport process in vascularized tissue in vivo. (Esther C, et al.,2011)
It is very important to reconstruct the graded vascular network, which can ensure the stable perfusion of the injured site of the implant. 3D bioprinting technology can accurately control the complex 3D structure and spatial distribution to create 3D vascularized tissue model, which has great application potential in drug toxicology, drug screening and potential disease modeling. For example, Massa et al. constructed a vascularized tissue engineering model using 3D bioprinting technology to simulate physiological drug diffusion and drug toxicity testing. Therefore, the prepared vascularized tissue can be used for in vitro research, drug screening and high-throughput detection in clinical trials of diseases such as cancer or ischemia (insufficient blood supply to organs or tissues), which can reduce the cost of drug development and provide important preclinical information.
Fig.2 Schematic diagram of vascularized liver structure. (Massa, S, et al.2017)
The biomanufacturing of clinically relevant volumes of living tissues and organs largely depends on the integration of vascular networks. Although the traditional biological manufacturing methods have made great progress, the construction of perfusion layered vascular network is still a major challenge. Our cell patterning technology for constructing vascularized tissue includes extrusion-based bioprinting, droplet-based bioprinting and laser-based bioprinting. These techniques can be used to manufacture specially designed tissue structures, create complex heterocellular structures with anatomical accuracy, and use bioactive factors that prolong angiogenesis to create vascular network patterns with the required geometry or size range, so as to be used together with other tissue components to create vascular system and bridge the gap between in vivo and in vitro drug test models.We can provide the following kinds of vascularized tissues:
We provide customers with cell patterning customization and related detection services based on Cell Patterning, you can contact our employees directly to ask questions if you are interested in our services, please contact us for more details.