Creative Bioarray provides you with in vitro cancer model based on Cell Patterning. This service can provide you with in vitro 3D cancer models with higher physiological relevance, so as to improve the predictive value of preclinical research and ultimately help you make decisions earlier in the development of cancer targeted therapy.
At present, one of the main factors leading to the low success rate of anticancer drug development is that preclinical models fail to fully summarize the complexity and heterogeneity of human cancer. For the reasons of flux and capacity, 2D monolayer tumor cell lines are often used in high-throughput screening growth inhibition analysis. However, the development of 2D monolayer tumor cell lines has inherent limitations, such as poor control of cell density, limited repeatability, low throughput, and in the case of co culture model, for cell types (such as cancer cells and stromal cells) The spatial distance between lacks replicable control. Therefore, the traditional 2D cell culture can't represent the in vivo environment. It is not suitable to evaluate the malignant ability of cancer cells or the expression of metastasis related genes, nor can it simulate the physiological factors conducive to cancer metastasis. Creative Bioarray's in vitro 3D cancer models can go beyond these limitations by integrating human cells, including patient derived cells, and allowing a series of experimental designs to accurately control each tumor microenvironment element. The models can improve our understanding of cancer pathology and develop better cancer therapies.
3D culture can control cell-cell interaction, cell polarity, gene expression and signal pathway, so 3D cancer models are very similar to tumor cells in vivo. In vitro 3D cancer models can accurately control each tumor microenvironment element by integrating human cells, including patient derived cells, and allowing a series of experimental designs. The role of tumor microenvironment in tumorigenesis, tumor progression, invasion and metastasis has been recognized, and now there is no doubt (Ham, Stephanie L, et al.,2016). In addition, tumor microenvironment is increasingly involved in regulating drug response and drug resistance. Therefore, the development of in vitro 3D cancer models can not only help to understand tumorigenesis, tumor progression, invasion and metastasis, but also greatly promote the development of therapeutic agents for tumor microenvironment mediated signals or tumor microenvironment compositions.
Fig1. Tumor is a complex heterocellular microenvironment (Ham, Stephanie L., et al.,2016).
Fig2. Tumor microenvironment regulates tumor metastasis and participates in all stages of tumor metastasis (Yuan, Y, et al.,2016)
3D cancer model can reproduce some important features of real solid tumor, so it is superior to traditional tumor cell culture. However, the traditional 3D tumor sphere is completely composed of tumor cells, which lack the participation of tumor stromal cells and the deposition of extracellular matrix is insufficient, so they can only partially simulate the in vivo conditions of tumor tissue. Creative Bioarray's 3D cancer models, which are composed of tumor cells and other cells, can better simulate the heterogeneous tumor microenvironment and the formation of natural connective tissue in vivo. Our 3D cancer models mainly include the following:
Creative Bioarray's 3D cancer models can perform cell-based detection in a simple and objective way in the tumor simulation microenvironment, providing you with valuable tools for cancer research and tumor drug discovery.
We provide customers with cell patterning customization and related detection services based on Cell Patterning, you can contact our employees directly to ask questions if you are interested in our services, please contact us for more details.