Using traditional culture tools to study cell behavior has shown great limitations. For example, in the process of conventional cell culture, the cell diffusion area and cell contraction force are often uncontrollable, and the surface properties of matrix materials and contact with cells are very uneven, which will affect the observation and regulation of cell behavior.
Creative Bioarray aims to provide you with contact guide wires through cell patterning technology to control the size and distribution of adhesive areas, so as to accurately control the cell shape and microenvironment, so as to facilitate you to regulate cell behavior under well controlled conditions.
Intercellular interactions are involved in many physiological and pathological processes, such as embryonic development, wound healing, tumor invasion and metastasis. Traditionally, cell interaction in vitro has been studied by randomly inoculating a variety of cell types on tissue culture matrix. However, using this method can't change the local cell inoculation density, nor control the degree of interaction between homotypic and heterotypic cells. Therefore, the cells cultured by this method are different from those in vivo in morphology, physiology and gene expression.
Our microfluidic cell patterning can reproduce the physiological cell culture environment in vivo, flexibly adjust the proportion and distance between different cells, accurately manipulate the degree of homotypic and heterotypic contact, and study the interaction between cells by controlling external conditions such as material concentration, solution temperature and pH value. It can reliably analyze key biological processes such as cell signal transduction and dynamic cell-cell interaction.
As an information readout, cell morphology is closely related to the basic physiological characteristics of cells, such as cell cycle progression, cell matrix adhesion, cell electrophysiological characteristics, cell differentiation, drug reactivity, aging, gene expression pattern and invasive potential. The change of cell shape will lead to the characteristic change of cell phenotype. Therefore, controlling cell morphology may become another mechanism to regulate cell function to promote human health. The traditional methods of regulating cell morphology are either attaching suspended cells to beads of different sizes or spreading cells on substrates coated with extracellular matrix of different densities. These methods lack repeatability.
Our cell patterning technology can accurately design the geometry and chemical properties of the substrate surface, control the size and distribution of cell adhesion area, and affect the cell morphology. Cell Patterning can help you better study how morphology and cytoskeleton structure affect cell structure and function.
Apoptosis has important biological significance in the fields of disease occurrence and drug action mechanism. Compared with the traditional biological apoptosis analysis technology, microfluidic cell patterning technology allows to simulate the microenvironment in vivo, control apoptosis and study intercellular signal transduction by regulating anisotropic cell morphology. The advantage of using cell patterning technology to study apoptosis is reflected in the flexible combination of cell culture, stimulation, detection and analysis modules, which can quickly realize the quantitative analysis of apoptosis information.
There are some challenges in culturing stem cells in vitro, including controlled proliferation while maintaining undifferentiated pluripotency and reliably guiding stem cell differentiation. At present, using traditional cell culture methods, it is difficult to meet these requirements and achieve a microenvironment similar to that in vivo. The emerging and rapid development of microfluidic cell patterning technology provides an ideal solution for simulating the microenvironment in vivo.
Through cell patterning, we can stimulate cells with biochemical clues, structural clues, mechanical stress and electromagnetic force, accurately simulate the shear stress in the physiological environment through the defined geometry and controlled perfusion flow rate, and control the cell microenvironment, including flow conditions, soluble factors and extracellular matrix, so as to guide stem cell differentiation.
Creative Bioarray provides customers with cell patterning customization and related detection services based on Cell Patterning, you can contact our employees directly to ask questions if you are interested in our services, please contact us for more details.